Mathematical modeling of apoptosis
© Schleich and Lavrik; licensee BioMed Central Ltd. 2013
Received: 6 March 2013
Accepted: 17 June 2013
Published: 26 June 2013
Apoptosis is a form of programmed cell death, which is fundamental to all multicellular organisms. Deregulation of apoptosis leads to a number of severe diseases including cancer. Apoptosis is initiated either by extrinsic signals via stimulation of receptors at the cellular surface or intrinsic signals, such as DNA damage or growth factor withdrawal. Apoptosis has been extensively studied using systems biology which substantially contributed to the understanding of this death signaling network. This review gives an overview of mathematical models of apoptosis and the potential of systems biology to contribute to the development of novel therapies for cancer or other apoptosis-related diseases.
KeywordsApoptosis Death receptors Systems biology Modeling Cancer
In this review we give an overview of systems biology approaches in apoptosis signaling with a focus on DR-induced apoptosis. Especially, we address a number of recent studies that provided new insights into apoptosis regulation since previous reviews have been written [11–14]. These studies include the determination of the DISC stoichiometry [15, 16], molecular determinants of type I/type II apoptotic signaling , novel statistical methods of parameter estimation and model discrimination of cell death models , and using mathematical modeling of apoptosis for the development of novel anticancer therapies [19–21].
Death receptor-induced apoptosis
DRs belong to the tumor necrosis factor receptor (TNF-R) family of proteins and initiate apoptosis upon stimulation with their respective ligands. All DRs are characterized by extracellular cysteine rich domains (CRD) as well as an intracellular approximately 80 amino acid long motif, the death domain (DD) [2, 22]. CD95 (Fas/APO-1), TNFR1, TRAIL receptor 1 (TRAIL-R1) and TRAIL-R2 comprise the best characterized DRs [2, 22, 23]. Other DRs are DR3 and DR6, EDA-R and NGF-R [2, 22, 24]. The CD95- and TRAIL-R-mediated pathways of apoptosis are among the best studied signaling pathways and have been extensively used in systems biology studies. Binding of the natural CD95 ligand (CD95L) or agonistic antibodies, e.g. anti-APO-1 , to CD95 initiates the signaling cascade. A first event in DR signaling is the recruitment of the adapter protein Fas-associated death domain (FADD) to oligomerized receptors  (Figure 1). Subsequently, initiator caspases-8 and −10 and cellular FLICE-inhibitory proteins (c-FLIP) are recruited to the complex, that is named DISC [26, 27] (Figure 1). Interactions between the molecules in the DISC are mediated by homotypic interactions. FADD is recruited via DD interactions with the receptor, caspase-8/10 and c-FLIP are recruited via death effector domain (DED) interactions with FADD . Procaspase-8 contains two DEDs in its N-terminal part followed by a large (~20 kDa) and small (~10 kDa) catalytic subunit . In the DISC procaspase-8 dimerizes, allowing the formation of an active site and activation of its catalytic activity [7, 26, 29–33]. The procaspase-8 dimer is further stabilized by cleavage between the large and small subunits [7, 33]. Subsequent cleavage between the prodomain and the large subunit releases active caspase-8 into the cytosol and initiates the apoptotic cascade . Caspase-8 activation is regulated by c-FLIP of which three isoforms are known: long (c-FLIPL), short (c-FLIPS) and Raji (c-FLIPR) [6, 34–37]. They have structural similarities to procaspase-8. All three c-FLIP isoforms have a tandem DED at their N-terminus . The long isoform, c-FLIPL, also has catalytically inactive caspase-like domains (p20, p12) [37, 38]. Both short isoforms block procaspase-8 processing and activation at the DISC [6, 27]. The long form, c-FLIPL, however, can accelerate procaspase-8 processing at low and intermediate concentrations, but inhibits it upon high concentrations [27, 29, 39]. Active caspase-8 further cleaves and activates the effector caspases-3 and −7 resulting in cell death  (Figure 1). In addition, it cleaves the pro-apoptotic Bcl-2 protein Bid which then translocates to the mitochondria triggering MOMP and amplification of the extrinsic signal [40, 41]. In CD95 signaling two types of cells are distinguished  (Figure 1). Type I cells form high amounts of DISC allowing efficient procaspases-8 and −3/7 activation without requiring amplification through the cleavage of Bid . Type II cells on the other hand activate caspase-8 at the DISC less efficiently and rely on the Bid-cleavage-mediated amplification loop . Thus, type I cells still die when MOMP is inhibited, but type II cells do not. Apoptosis is further regulated by two other protein families. Inhibitors of Apoptosis proteins (IAP), with currently six human members: XIAP, c-IAP1, c-IAP2, NAIP, Bruce and survivin, can directly inhibit caspases . The IAP family members share a conserved sequence motif, the baculoviral IAP repeat (BIR) domain, which is required for their function [7, 44]. The family of Bcl-2 proteins has crucial functions in regulating MOMP with pro-apoptotic (e.g. Bax, Bak, Bid) and anti-apoptotic (e.g. Bcl-2, Bcl-XL) members [40, 45]. Bcl-2 family members are characterized by the presence of the so-called Bcl-2 homology domain (BH1-BH4) [40, 45].
Although best known as a ‘death receptor’, there is increasing evidence that CD95 also has functions in non-apoptotic signaling, such as tumor growth and invasion, proliferation and necroptosis [46–52]. It has been shown by numerous groups that CD95 activates NF-κB, a central mediator of inflammation, and MAPK signaling [46, 53–59] (Figure 1).
Mathematical formalisms used in systems biology
In systems biology experimental approaches are combined with mathematical modeling to understand complex behavior of cells and organisms. Experimental approaches and mathematical models are connected through a cyclic workflow . Experimental data is used as input for mathematical models that, in turn, generate biological predictions. These predictions are then again verified by experimental approaches, thus completing the cycle. Experimental approaches, used in systems biology of apoptosis so far, include quantitative Western Blot, cell death assays, single cell analysis and mass spectrometry.
Numerous different modeling formalisms can be used to describe cellular systems and have been used, in particular, for modeling apoptosis. We shall briefly present the major modeling formalisms below.
In Boolean modeling protein-protein interactions are described qualitatively and kinetics of individual protein interactions are not taken into account. The signaling network is represented as a graph, signaling molecules are represented by the nodes and interactions by edges . Each node can be either in an ‘off’ or ‘on’ state, represented by 0 and 1, respectively . The state of each node depends on the state of its input nodes governed by logic rules (so-called operators or gates). Common boolean rules are the AND, OR and NOT operators. The AND operator will switch a node to the ‘on’ state only if all input nodes were in the ‘on’ state. On the contrary, an OR operator will switch a node to the ‘on’ state if one of the input nodes is in the ‘on’ state. The NOT operator is only used together with the other operators and is true if the corresponding input node is in the ‘off’ state . Since each signaling component of the network can be only in two different states, boolean modeling cannot simulate temporal dynamics of protein concentrations.
Such systems of ODEs can be solved numerically using standard computer software (e.g. Matlab). Usually dynamical pathway models include a large number of kinetic parameters which are not known and mostly cannot be measured experimentally. Therefore, parameters are estimated based on experimental data, e.g. kinetics of protein concentrations in the pathway of interest. Parameters can be estimated using the method of least squares . Due to the high number of kinetic parameters compared to little experimental data, model fitting can yield multiple parameter values that can describe the data equally well. In order to test the robustness of a model sensitivity analysis is applied [61, 63]. Furthermore, statistical methods can be used for parameter estimation of ODE models and are especially useful for discrimination between different model topologies with equally good fit to the same dataset . ODEs assume homogenous distribution of interacting molecules and neglect spatial information. In order to take into account diffusion of molecules partial differential equations (PDEs) can be used .
Contrary to deterministic models, e.g. ODEs, stochastic models are based on the likelihood that two molecules interact. Another modeling approach is agent-based modeling [65–69]. This approach is a rules-based modeling approach in which each component of the model system is represented by an agent with a certain behavior based on its biological functions.
Mathematical modeling of apoptosis
Mathematical modeling of apoptosis signaling reaches back more than a decade ago when Fussenegger et al. generated the first model of apoptosis . This model was based on ODEs and described the apoptotic pathway both by extrinsic and intrinsic signals, but was not based on experimental data. The entirely theoretical model by Fussenegger et al. was followed by a number of ODE-based models supported by experimental data. The first model of this type described on the quantitative level the extrinsic apoptotic pathway, namely CD95 signaling . This first experiment-based model of apoptosis initiated many more ODE-models of both the extrinsic and intrinsic pathways that appeared within the last decade. In addition, bistability of DR-induced apoptosis depending on initial procaspase-8 molecule numbers was demonstrated using an ODE model [72, 73]. Besides ODE-based models other approaches have been applied to apoptosis modeling, including boolean modeling [74–77], Bayesian modeling , petri nets , cellular automata [80, 81] and agent-based modeling .
Various mathematical formalisms applied to apoptotic signaling allowed to address a number of biological questions, e.g. how the switching between life and death in the cell occurs. In particular, it was possible to understand the contribution of different apoptosis regulators to apoptosis induction. For instance, considering extrinsic apoptosis signaling one might ask what the cell death stage-limiting steps are: DISC formation, activation of effector caspases, or cleavage of the caspase substrates. By combining quantitative Western Blot with a structured information ODE model our group revealed a threshold mechanism in CD95-initiated apoptosis which could also be verified experimentally [71, 82]. In this work it was shown that activation of caspase-8 at the DISC is a stage-limiting step in extrinsic apoptosis in type I cells. Furthermore, it was shown that efficient DISC formation is the central control point of extrinsic apoptosis and the amount of the caspase-8 inhibitors c-FLIP in the cell is the major factor defining life/death decisions.
Similar questions, e.g. what the major regulators are and how the threshold is defined, were addressed using ODE modeling of intrinsic apoptosis. Models of the intrinsic apoptotic pathway demonstrated a crucial role of XIAP in caspase activation via the apoptosome [73, 83]. Rehm et al. demonstrated that XIAP inhibits caspase activation only above a certain threshold, but enhances it at low concentrations . Based on this model a simulation tool called APOPTO-CELL is available online .
The fascinating insights into the molecular mechanisms of apoptotic signaling were achieved by the combination of single cell analysis with mathematical modeling. For instance, spatio-temporal activation of caspases in TRAIL-induced apoptosis was understood in type II cells. It was shown that initiator caspases are active in the pre-MOMP phase while effector caspases are only active after MOMP . In another model the same group analyzed cell-to-cell variability upon TRAIL stimulation . They compared sister cells using time-lapse microscopy and FRET-based caspase-activity reporters and found that differences in signaling outcome are due to variations in protein levels.
Mathematical modeling of apoptosis also allowed determining and supporting quite some molecular paradigms. For a while the differences between type I and type II cells was an issue of debate. In a recent study Aldridge et al. studied the molecular determinants of type I vs. type II cells and demonstrated that the ratio of caspase-3 to XIAP is one of the major determinants of type I/type II decisions . Another question that was unclear for a number of years is whether c-FLIPL could indeed activate caspase-8 at the DISC. Only mathematical modeling was capable to predict the exact concentrations of the DISC components when c-FLIPL could play an activating role . These predictions were successfully confirmed by experimental data. Finally, a new mechanism of apoptosis signaling has been discovered recently using biochemical analysis, mass spectrometry and mathematical modeling. Namely, our group and the group of Marion MacFarlane determined the stoichiometry of the CD95 and TRAIL-R DISCs and found that caspase-8 outnumbers FADD at the DISC forming the so-called caspase-8 chains [15, 16]. Using mathematical modeling we further analyzed the dynamics of the chain formation and found that the DISC is a very dynamic system and its stoichiometry, i.e. length of the caspase-8 chains, is defined by the strength of CD95 stimulation . This finding adds another layer of complexity in caspase-8 activation and regulation of apoptosis in extrinsic signaling.
The non-apoptotic signaling features of CD95 have gained increasing interest, and, consequently, the concept of switching the phenotypes between apoptotic and non-apoptotic signaling also attracted the attention of systems biology research. To address the switch between apoptosis, necroptosis and survival Calzone et al. developed a Boolean model of TNF-R and CD95 signaling . This was the first model of necroptosis that described the switch between necroptosis, apoptosis and survival as well as established the major molecules responsible for necroptosis induction. The other challenging question is how stimulation of DRs could also result in the induction of non-apoptotic pathways, such as NF-κB and MAPK pathways. Our ODE models of CD95-induced apoptosis found that DISC formation also leads to the induction of NF-κB and MAPK pathways [55, 57]. Intriguingly, caspase-8 activity and the concentration of c-FLIP play an important role in NF-κB and MAPK induction [55, 57]. Thus, our systems biology studies have demonstrated that the amounts of the main regulators of DR-induced apoptosis procaspase-8 and c-FLIP at the DISC also play a key role in the induction of non-apoptotic signaling.
After creating the basic models of the apoptosis pathways, currently systems biology of cell death is entering a next very important phase. The central question now is how defects in apoptotic networks are connected to various diseases and how apoptosis models could be used to develop better treatments. Especially important are opportunities that are provided by systematic consideration of the apoptotic network and possible connections to personalized medicine.
Apoptosis plays a central role in development and prevention of apoptosis is a hallmark of cancer. Despite extremely detailed understanding of the apoptotic pathway little progress has been made with respect to cancer therapy. Chemotherapy, radiotherapy and surgery remain the best treatment options so far. Systems biology studies of apoptosis may play an important role in the development of novel anticancer therapies. Indeed, recent studies from different groups used mathematical models of apoptosis and applied them to cancer cells [19–21]. This allowed finding out the major determinants of apoptosis resistance in cancer cells, which, in turn, provides a basis for the development of novel anti-cancer therapies targeting key components of the apoptotic pathway [19–21]. These first studies show ample opportunities that systems biology of the apoptotic network might provide with respect to cancer treatment and open new avenues for drug development.
Systems biology of apoptosis in the last decade has undergone a tremendous development from the first theoretical model of apoptosis to models directly connecting defects in apoptosis networks to cancer. The next issues to address are the dynamics of apoptosis networks and cross-talk to other signaling pathways at the level of the cell, tissue and organism. A very ambitious question to address is what the role of the apoptotic network is in the diseases of the century e.g. ageing and metabolic diseases. Future challenges also involve further development of models of diseases associated to defects in apoptosis and successful development of anti-cancer therapies based on systems biology models.
We acknowledge the Ministry of Sciences and Economic Affairs of Saxony-Anhalt (Research Centre Dynamic Systems: Biosystems Engineering, MW -21LMS 5), BMBF (eBIO project “ImmunoQuant” - TPU - 0316170G) and Helmholtz-Russia Joint Research Groups—2008-2 for supporting our work.
- Thompson CB: Apoptosis in the pathogenesis and treatment of disease. Science. 1995, 267: 1456-1462. 10.1126/science.7878464.PubMedView Article
- Krammer PH, Arnold R, Lavrik IN: Life and death in peripheral T cells. Nat Rev Immunol. 2007, 7: 532-542. 10.1038/nri2115.PubMedView Article
- Kischkel FC, Hellbardt S, Behrmann I, Germer M, Pawlita M, Krammer PH, Peter ME: Cytotoxicity-dependent APO-1 (Fas/CD95)-associated proteins form a death-inducing signaling complex (DISC) with the receptor. EMBO J. 1995, 14: 5579-5588.PubMed CentralPubMed
- Rodriguez J, Lazebnik Y: Caspase-9 and APAF-1 form an active holoenzyme. Genes Dev. 1999, 13: 3179-3184. 10.1101/gad.13.24.3179.PubMed CentralPubMedView Article
- Robertson JD, Orrenius S, Zhivotovsky B: Review: nuclear events in apoptosis. J Struct Biol. 2000, 129: 346-358. 10.1006/jsbi.2000.4254.PubMedView Article
- Golks A, Brenner D, Fritsch C, Krammer PH, Lavrik IN: c-FLIPR, a new regulator of death receptor-induced apoptosis. J Biol Chem. 2005, 280: 14507-14513. 10.1074/jbc.M414425200.PubMedView Article
- Fuentes-Prior P, Salvesen GS: The protein structures that shape caspase activity, specificity, activation and inhibition. Biochem J. 2004, 384: 201-232. 10.1042/BJ20041142.PubMed CentralPubMedView Article
- Kitano H: Computational systems biology. Nature. 2002, 420: 206-210. 10.1038/nature01254.PubMedView Article
- Kitano H: Systems biology: a brief overview. Science. 2002, 295: 1662-1664. 10.1126/science.1069492.PubMedView Article
- Kitano H: Foundations of Systems Biology. 2001, Cambridge: Mit Press
- Lavrik IN: Systems biology of apoptosis signaling networks. Curr Opin Biotechnol. 2010, 21: 551-555. 10.1016/j.copbio.2010.07.001.PubMedView Article
- Lavrik IN, Eils R, Fricker N, Pforr C, Krammer PH: Understanding apoptosis by systems biology approaches. Mol Biosyst. 2009, 5: 1105-1111. 10.1039/b905129p.PubMedView Article
- Huber H, Bullinger E, Rehm M: Systems Biology Approaches to the Study of Apoptosis. Essentials of Apoptosis. Edited by: Dong Z, Yin X-M. 2009, New York, NY: Humana Press, 283-297. 2View Article
- Zhivotovsky B, Barillot E: Systems Biology Analysis of Cell Death Pathways in Cancer: How Collaborative and Interdisciplinary Research Helps. Cancer Systems Biology, Bioinformatics and Medicine. Edited by: Cesario A, Marcus F. 2011, Springer Netherlands: Dordrecht
- Schleich K, Warnken U, Fricker N, Oztürk S, Richter P, Kammerer K, Schnölzer M, Krammer PH, Lavrik IN: Stoichiometry of the CD95 death-inducing signaling complex: experimental and modeling evidence for a death effector domain chain model. Mol Cell. 2012, 47: 306-319. 10.1016/j.molcel.2012.05.006.PubMedView Article
- Dickens LS, Boyd RS, Jukes-Jones R, Hughes MA, Robinson GL, Fairall L, Schwabe JWR, Cain K, MacFarlane M: A Death Effector Domain Chain DISC Model Reveals a Crucial Role for Caspase-8 Chain Assembly in Mediating Apoptotic Cell Death. Mol Cell. 2012, 47: 291-305. 10.1016/j.molcel.2012.05.004.PubMed CentralPubMedView Article
- Aldridge BB, Gaudet S, Lauffenburger DA, Sorger PK: Lyapunov exponents and phase diagrams reveal multi-factorial control over TRAIL-induced apoptosis. Mol Syst Biol. 2011, 7: 553-PubMed CentralPubMedView Article
- Eydgahi H, Chen WW, Muhlich JL, Vitkup D, Tsitsiklis JN, Sorger PK: Properties of cell death models calibrated and compared using Bayesian approaches. Mol Syst Biol. 2013, 9: 644-PubMed CentralPubMedView Article
- Hector S, Rehm M, Schmid J, Kehoe J, McCawley N, Dicker P, Murray F, McNamara D, Kay EW, Concannon CG, et al: Clinical application of a systems model of apoptosis execution for the prediction of colorectal cancer therapy responses and personalisation of therapy. Gut. 2012, 61: 725-733. 10.1136/gutjnl-2011-300433.PubMedView Article
- Huber HJ, Dussmann H, Kilbride SM, Rehm M, Prehn JHM: Glucose metabolism determines resistance of cancer cells to bioenergetic crisis after cytochrome-c release. Mol Syst Biol. 2011, 7: 470-PubMed CentralPubMedView Article
- Lee MJ, Ye AS, Gardino AK, Heijink AM, Sorger PK, MacBeath G, Yaffe MB: Sequential Application of Anticancer Drugs Enhances Cell Death by Rewiring Apoptotic Signaling Networks. Cell. 2012, 149: 780-794. 10.1016/j.cell.2012.03.031.PubMed CentralPubMedView Article
- Ashkenazi A, Dixit VM: Death Receptors: Signaling and Modulation. Science. 1998, 281: 1305-1308.PubMedView Article
- Krammer PH: CD95's deadly mission in the immune system. Nature. 2000, 407: 789-795. 10.1038/35037728.PubMedView Article
- Lavrik I, Golks A, Krammer PH: Death receptor signaling. J Cell Sci. 2005, 118: 265-267. 10.1242/jcs.01610.PubMedView Article
- Trauth BC, Klas C, Peters AM, Matzku S, Möller P, Falk W, Debatin KM, Krammer PH: Monoclonal antibody-mediated tumor regression by induction of apoptosis. Science. 1989, 245: 301-305. 10.1126/science.2787530.PubMedView Article
- Medema JP, Scaffidi C, Kischkel FC, Shevchenko A, Mann M, Krammer PH, Peter ME: FLICE is activated by association with the CD95 death-inducing signaling complex (DISC). EMBO J. 1997, 16: 2794-2804. 10.1093/emboj/16.10.2794.PubMed CentralPubMedView Article
- Krueger A, Schmitz I, Baumann S, Krammer PH, Kirchhoff S: Cellular FLICE-inhibitory protein splice variants inhibit different steps of caspase-8 activation at the CD95 death-inducing signaling complex. J Biol Chem. 2001, 276: 20633-20640. 10.1074/jbc.M101780200.PubMedView Article
- Park HH, Lo Y-C, Lin S-C, Wang L, Yang JK, Wu H: The death domain Superfamily in Intracellular Signaling of Apoptosis and Inflammation. Annu Rev Immunol. 2007, 25: 561-586. 10.1146/annurev.immunol.25.022106.141656.PubMed CentralPubMedView Article
- Chang DW, Xing Z, Pan Y, Algeciras-Schimnich A, Barnhart BC, Yaish-Ohad S, Peter ME, Yang X: c-FLIP(L) is a dual function regulator for caspase-8 activation and CD95-mediated apoptosis. EMBO J. 2002, 21: 3704-3714. 10.1093/emboj/cdf356.PubMed CentralPubMedView Article
- Golks A, Brenner D, Schmitz I, Watzl C, Krueger A, Krammer PH, Lavrik IN: The role of CAP3 in CD95 signaling: new insights into the mechanism of procaspase-8 activation. Cell Death Differ. 2006, 13: 489-498. 10.1038/sj.cdd.4401766.PubMedView Article
- Hughes MA, Harper N, Butterworth M, Cain K, Cohen GM, MacFarlane M: Reconstitution of the death-inducing signaling complex reveals a substrate switch that determines CD95-mediated death or survival. Mol Cell. 2009, 35: 265-279. 10.1016/j.molcel.2009.06.012.PubMedView Article
- Yu JW, Jeffrey PD, Shi Y: Mechanism of procaspase-8 activation by c-FLIPL. Proc Natl Acad Sci U S A. 2009, 106: 8169-8174. 10.1073/pnas.0812453106.PubMed CentralPubMedView Article
- Lavrik IN, Golks A, Krammer PH: Caspases: pharmacological manipulation of cell death. J Clin Invest. 2005, 115: 2665-2672. 10.1172/JCI26252.PubMed CentralPubMedView Article
- Scaffidi C, Schmitz I, Krammer PH, Peter ME: The role of c-FLIP in modulation of CD95-induced apoptosis. J Biol Chem. 1999, 274: 1541-1548. 10.1074/jbc.274.3.1541.PubMedView Article
- Golks A, Brenner D, Krammer PH, Lavrik IN: The c-FLIP-NH2 terminus (p22-FLIP) induces NF-kappaB activation. J Exp Med. 2006, 203: 1295-1305. 10.1084/jem.20051556.PubMed CentralPubMedView Article
- Ueffing N, Schuster M, Keil E, Schulze-Osthoff K, Schmitz I: Up-regulation of c-FLIP short by NFAT contributes to apoptosis resistance of short-term activated T cells. Blood. 2008, 112: 690-698. 10.1182/blood-2008-02-141382.PubMedView Article
- Oztürk S, Schleich K, Lavrik IN: Cellular FLICE-like inhibitory proteins (c-FLIPs): Fine-tuners of life and death decisions. Exp Cell Res. 2012, 318: 1324-1331. 10.1016/j.yexcr.2012.01.019.PubMedView Article
- Lavrik IN, Krammer PH: Regulation of CD95/Fas signaling at the DISC. Cell Death Differ. 2012, 19: 36-41. 10.1038/cdd.2011.155.PubMed CentralPubMedView Article
- Fricker N, Beaudouin J, Richter P, Eils R, Krammer PH, Lavrik IN: Model-based dissection of CD95 signaling dynamics reveals both a pro- and antiapoptotic role of c-FLIPL. J Cell Biol. 2010, 190: 377-389. 10.1083/jcb.201002060.PubMed CentralPubMedView Article
- Yan N, Shi Y: Mechanisms of apoptosis through structural biology. Annu Rev Cell Dev Biol. 2005, 21: 35-56. 10.1146/annurev.cellbio.21.012704.131040.PubMedView Article
- Li H, Zhu H, Xu C, Yuan J: Cleavage of BID by Caspase 8 Mediates the Mitochondrial Damage in the Fas Pathway of Apoptosis. Cell. 1998, 94: 491-501. 10.1016/S0092-8674(00)81590-1.PubMedView Article
- Barnhart BC, Alappat EC, Peter ME: The CD95 Type I/Type II model. Semin Immunol. 2003, 15: 185-193. 10.1016/S1044-5323(03)00031-9.PubMedView Article
- Deveraux QL, Reed JC: IAP family proteins–suppressors of apoptosis. Genes Dev. 1999, 13: 239-252. 10.1101/gad.13.3.239.PubMedView Article
- Shi Y: Mechanisms of Caspase Activation and Inhibition during Apoptosis. Mol Cell. 2002, 9: 459-470. 10.1016/S1097-2765(02)00482-3.PubMedView Article
- Adams JM, Cory S: The Bcl-2 Protein Family: Arbiters of Cell Survival. Science. 1998, 281: 1322-1326.PubMedView Article
- Lee S-M, Kim E-J, Suk K, Lee W-H: Stimulation of Fas (CD95) induces production of pro-inflammatory mediators through ERK/JNK-dependent activation of NF-κB in THP-1 cells. Cell Immunol. 2011, 271: 157-162. 10.1016/j.cellimm.2011.06.019.PubMedView Article
- Chen L, Park S-M, Tumanov AV, Hau A, Sawada K, Feig C, Turner JR, Fu Y-X, Romero IL, Lengyel E, Peter ME: CD95 promotes tumour growth. Nature. 2010, 465: 492-496. 10.1038/nature09075.PubMed CentralPubMedView Article
- Steller EJ, Ritsma L, Raats DAE, Hoogwater FJH, Emmink BL, Govaert KM, Laoukili J, Borel Rinkes IHM, Van Rheenen J, Kranenburg O: The death receptor CD95 activates the cofilin pathway to stimulate tumour cell invasion. EMBO Rep. 2011, 12: 931-937. 10.1038/embor.2011.129.PubMed CentralPubMedView Article
- Choi K, Ni L, Jonakait GM: Fas ligation and tumor necrosis factor alpha activation of murine astrocytes promote heat shock factor-1 activation and heat shock protein expression leading to chemokine induction and cell survival. J Neurochem. 2011, 116: 448-View Article
- Tang D, Lotze MT, Kang R, Zeh HJ: Apoptosis promotes early tumorigenesis. Oncogene. 2011, 30: 1851-1854. 10.1038/onc.2010.573.PubMedView Article
- Strasser A, Jost PJ, Nagata S: The many roles of FAS receptor signaling in the immune system. Immunity. 2009, 30: 180-192. 10.1016/j.immuni.2009.01.001.PubMed CentralPubMedView Article
- Geserick P, Hupe M, Moulin M, Wong WW-L, Feoktistova M, Kellert B, Gollnick H, Silke J, Leverkus M: Cellular IAPs inhibit a cryptic CD95-induced cell death by limiting RIP1 kinase recruitment. J Cell Biol. 2009, 187: 1037-1054. 10.1083/jcb.200904158.PubMed CentralPubMedView Article
- Shikama Y, Yamada M, Miyashita T: Caspase-8 and caspase-10 activate NF-kappaB through RIP, NIK and IKKalpha kinases. Eur J Immunol. 2003, 33: 1998-2006. 10.1002/eji.200324013.PubMedView Article
- Van Raam BJ, Salvesen GS: Proliferative versus apoptotic functions of caspase-8 Hetero or homo: The caspase-8 dimer controls cell fate. Biochim Biophys Acta. 2012, 1824: 113-122. 10.1016/j.bbapap.2011.06.005.PubMed CentralPubMedView Article
- Neumann L, Pforr C, Beaudouin J, Pappa A, Fricker N, Krammer PH, Lavrik IN, Eils R: Dynamics within the CD95 death-inducing signaling complex decide life and death of cells. Mol Syst Biol. 2010, 6: 352-PubMed CentralPubMedView Article
- Nakajima A, Kojima Y, Nakayama M, Yagita H, Okumura K, Nakano H: Downregulation of c-FLIP promotes caspase-dependent JNK activation and reactive oxygen species accumulation in tumor cells. Oncogene. 2008, 27: 76-84. 10.1038/sj.onc.1210624.PubMedView Article
- Kober AMM, Legewie S, Pforr C, Fricker N, Eils R, Krammer PH, Lavrik IN: Caspase-8 activity has an essential role in CD95/Fas-mediated MAPK activation. Cell Death & Disease. 2011, 2: e212-10.1038/cddis.2011.93.View Article
- Kataoka T, Budd RC, Holler N, Thome M, Martinon F, Irmler M, Burns K, Hahne M, Kennedy N, Kovacsovics M, Tschopp J: The caspase-8 inhibitor FLIP promotes activation of NF-κB and Erk signaling pathways. Curr Biol. 2000, 10: 640-648. 10.1016/S0960-9822(00)00512-1.PubMedView Article
- Kreuz S, Siegmund D, Rumpf J-J, Samel D, Leverkus M, Janssen O, Häcker G, Dittrich-Breiholz O, Kracht M, Scheurich P, Wajant H: NFkappaB activation by Fas is mediated through FADD, caspase-8, and RIP and is inhibited by FLIP. J Cell Biol. 2004, 166: 369-380. 10.1083/jcb.200401036.PubMed CentralPubMedView Article
- Saadatpour A, Albert R: Discrete dynamic modeling of signal transduction networks. Computational Modeling of Signaling Networks. Edited by: Liu X, Betterton MD. 2012, Totowa, NJ: Humana Press, 255-272.View Article
- Chen WW, Niepel M, Sorger PK: Classic and contemporary approaches to modeling biochemical reactions. Genes Dev. 2010, 24: 1861-1875. 10.1101/gad.1945410.PubMed CentralPubMedView Article
- Spencer SL, Sorger PK: Measuring and modeling apoptosis in single cells. Cell. 2011, 144: 926-939. 10.1016/j.cell.2011.03.002.PubMed CentralPubMedView Article
- Lavrik IN: Systems Biology of Apoptosis. 2013, New York: Springer, 1View Article
- Waage P, Guldberg CM: Studies concerning affinity. J Chem Educ. 1986, 63: 1044-10.1021/ed063p1044.View Article
- Bonabeau E: Agent-based modeling: methods and techniques for simulating human systems. Proc Natl Acad Sci U S A. 2002, 99 (Suppl 3): 7280-7287.PubMed CentralPubMedView Article
- Macal CM, North MJ: Agent-based modeling and simulation. Proceedings of the 2009 Winter Simulation Conference (WSC). 2009, 86-98.View Article
- Macal CM, North MJ: Tutorial on agent-based modelling and simulation. Journal of Simulation. 2010, 4: 151-162. 10.1057/jos.2010.3.View Article
- Brown BN, Price IM, Toapanta FR, Dealmeida DR, Wiley CA, Ross TM, Oury TD, Vodovotz Y: An agent-based model of inflammation and fibrosis following particulate exposure in the lung. Math Biosci. 2011, 231: 186-196. 10.1016/j.mbs.2011.03.005.PubMed CentralPubMedView Article
- Roche B, Drake JM, Rohani P: An Agent-Based Model to study the epidemiological and evolutionary dynamics of Influenza viruses. BMC Bioinformatics. 2011, 12: 87-10.1186/1471-2105-12-87.PubMed CentralPubMedView Article
- Fussenegger M, Bailey JE, Varner J: A mathematical model of caspase function in apoptosis. Nat Biotechnol. 2000, 18: 768-774. 10.1038/77589.PubMedView Article
- Bentele M, Lavrik I, Ulrich M, Stösser S, Heermann DW, Kalthoff H, Krammer PH, Eils R: Mathematical modeling reveals threshold mechanism in CD95-induced apoptosis. J Cell Biol. 2004, 166: 839-851. 10.1083/jcb.200404158.PubMed CentralPubMedView Article
- Eissing T, Conzelmann H, Gilles ED, Allgöwer F, Bullinger E, Scheurich P: Bistability analyses of a caspase activation model for receptor-induced apoptosis. J Biol Chem. 2004, 279: 36892-36897. 10.1074/jbc.M404893200.PubMedView Article
- Legewie S, Blüthgen N, Herzel H: Mathematical modeling identifies inhibitors of apoptosis as mediators of positive feedback and bistability. PLoS Comput Biol. 2006, 2: e120-10.1371/journal.pcbi.0020120.PubMed CentralPubMedView Article
- Saez-Rodriguez J, Alexopoulos LG, Epperlein J, Samaga R, Lauffenburger DA, Klamt S, Sorger PK: Discrete logic modelling as a means to link protein signalling networks with functional analysis of mammalian signal transduction. Mol Syst Biol. 2009, 5: 331-PubMed CentralPubMedView Article
- Schlatter R, Schmich K, Avalos Vizcarra I, Scheurich P, Sauter T, Borner C, Ederer M, Merfort I, Sawodny O: ON/OFF and beyond--a boolean model of apoptosis. PLoS Comput Biol. 2009, 5: e1000595-10.1371/journal.pcbi.1000595.PubMed CentralPubMedView Article
- Mai Z, Liu H: Boolean network-based analysis of the apoptosis network: irreversible apoptosis and stable surviving. J Theor Biol. 2009, 259: 760-769. 10.1016/j.jtbi.2009.04.024.PubMedView Article
- Calzone L, Tournier L, Fourquet S, Thieffry D, Zhivotovsky B, Barillot E, Zinovyev A: Mathematical modelling of cell-fate decision in response to death receptor engagement. PLoS Comput Biol. 2010, 6: e1000702-10.1371/journal.pcbi.1000702.PubMed CentralPubMedView Article
- Yang ZR: Prediction of caspase cleavage sites using Bayesian bio-basis function neural networks. Bioinformatics. 2005, 21: 1831-1837. 10.1093/bioinformatics/bti281.PubMedView Article
- Heiner M, Koch I, Will J: Model validation of biological pathways using Petri nets–demonstrated for apoptosis. Bio Systems. 2004, 75: 15-28. 10.1016/j.biosystems.2004.03.003.PubMedView Article
- Apte A, Bonchev D, Fong S: Cellular automata modeling of FASL-initiated apoptosis. Chem Biodivers. 2010, 7: 1163-1172. 10.1002/cbdv.200900422.PubMedView Article
- Chen C, Cui J, Lu H, Wang R, Zhang S, Shen P: Modeling of the role of a Bax-activation switch in the mitochondrial apoptosis decision. Biophys J. 2007, 92: 4304-4315. 10.1529/biophysj.106.099606.PubMed CentralPubMedView Article
- Lavrik IN, Golks A, Riess D, Bentele M, Eils R, Krammer PH: Analysis of CD95 threshold signaling: triggering of CD95 (FAS/APO-1) at low concentrations primarily results in survival signaling. J Biol Chem. 2007, 282: 13664-13671. 10.1074/jbc.M700434200.PubMedView Article
- Rehm M, Huber HJ, Dussmann H, Prehn JHM: Systems analysis of effector caspase activation and its control by X-linked inhibitor of apoptosis protein. EMBO J. 2006, 25: 4338-4349. 10.1038/sj.emboj.7601295.PubMed CentralPubMedView Article
- Huber HJ, Rehm M, Plchut M, Düssmann H, Prehn JHM: APOPTO-CELL–a simulation tool and interactive database for analyzing cellular susceptibility to apoptosis. Bioinformatics. 2007, 23: 648-650. 10.1093/bioinformatics/btl684.PubMedView Article
- Albeck JG, Burke JM, Aldridge BB, Zhang M, Lauffenburger DA, Sorger PK: Quantitative analysis of pathways controlling extrinsic apoptosis in single cells. Mol Cell. 2008, 30: 11-25. 10.1016/j.molcel.2008.02.012.PubMed CentralPubMedView Article
- Spencer SL, Gaudet S, Albeck JG, Burke JM, Sorger PK: Non-genetic origins of cell-to-cell variability in TRAIL-induced apoptosis. Nature. 2009, 459: 428-432. 10.1038/nature08012.PubMed CentralPubMedView Article
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.